Nucleic Acid Hybridization with RNA Immobilized on Filter Paper

Nucleic acid hybridization with RNA immobilized on filter paper.

RNA has been immobilized in a manner suitable for use in molecular hybridization experiments with dissolved RNA or DNA by a nonaqueous solid-phase reaction with carbonyldiimidazole and RNA "dry coated" on cellulose or, preferably, on previously activated phosphocellulose filters. Immobilization of RNA does not appear to alter its chemical character or cause it to acquire affinity for unspecific...

Hybridization properties of immobilized nucleic acids

The 5'-end attachment of oligonucleotides to dextran supports facilitates the study of the hybridization properties of an immobilized oligonucleotide system. The hybridization properties which were studied include: hybridization capacity and kinetics, hybridization-complex stability, and reagents influencing hybridization efficiency. Results of these experiments reveal that the hybridization ef...

Studies on nucleic acid reassociation kinetics: retarded rate of hybridization of RNA with excess DNA.

The rate of reaction of excess double-stranded bacteriophage phiX174 and plasmid RSF2124 DNA drivers with enzymatically synthesized asymmetric RNA tracers was measured. Other reactions were carried out with excess Escherichia coli DNA and E. coli RNA labeled in vivo. RNA and DNA fragment lengths were held approximately equal. For each case it was shown that in DNA excess the rate constant for R...

8.5 Detecting Nucleic Acid Hybridization

The double-helical structure of nucleic acids, in which one strand binds specifically to its exact complement, could not have been designed to be more useful for the study of biology. The ease of designing assays based on this feature is responsible for the incredibly fast pace that has characterized molecular biology research since its inception. With the incorporation of fluorescence technolo...

Nucleic Acid Hybridization – General Aspects